isolation and purification of echinococcus granulosus antigen b from hydatid cyst fluid using three different methods

Isolation and purification of Echinococcus granulosus antigen B from hydatid cyst fluid using three different methods

Hydatid cyst, the larval stage of cestodes Echinococcus spp., is recognized as a zoonotic infection in the world. The World Health Organization (WHO) has recently classified echinococcosis in a group of neglected tropical diseases.The prevalence of Echinococcus granulosus infection is high in Iran due to the presence of various intermediate hosts in this country. Considering the rising trend of...

Chemical Composition of Hydatid Cyst Fluid of Echinococcus granulosus

Chemical Composition of Hydatid Cyst Fluid of Echinococcus granulosus

Sumnuuy The fluid of hydatid cysts occurring in the liver and the lung have been analysed for proteins, carbohydrates, nitrogenow waste products, lipids, electrolytes and nucleic acids contents. The data obtained from the fluid of the liver cysts were compared with those obtained from the lung cysts. Furthermore, the same analyses were carried out to investigate what kind of changes, if any, oc...

Antibody response to glycan antigens of hydatid cyst fluid, laminated layer and protoscolex of Echinococcus granulosus

Background: Hydatid disease is characterized by long-term growth of hydatid cysts in the human. The glycan antigens have an important role in the immunology of hydatid cyst. In this study immunological reaction of host sera to different glycan antigens of the cyst, has been investigated. Methods: The antibody responses were tested to glycoprotein and glycolipid of the laminated layer (LL), c...

An Easy and Efficient Method for Native and Immunoreactive Echinococcus granulosus Antigen 5 Enrichment from Hydatid Cyst Fluid

BACKGROUND Currently, the serodiagnosis of cystic echinococcosis relies mostly on crude Echinococcus granulosus hydatid cyst fluid as the antigen. Consequently, available immunodiagnostic tests lack standardization of the target antigen and, in turn, this is reflected on poor sensitivity and specificity of the serological diagnosis. METHODOLOGY/PRINCIPAL FINDINGS Here, a chromatographic metho...

Clinical evaluation of a complement fixation test using human hydatid cyst fluid antigen for the detection of Echinococcus granulosus antibody

Complement fixation (CF) is a commonly used serodiagnostic test for hydatid disease, especially recommended for the evaluation of cure after surgery or for checking on treatment with antihelminthic drugs (9). However, the absence of an FDA-approved standardized antigen makes it difficult to assess the performance of different CF tests by using published studies. The objectives of this study wer...